Geobacillus stearothermophilus (formally Bacillus stearothermophilus) is a rod-shaped, incubated. A color and/or turbidity change indicates the results of the sterilization process; no change indicates that the sterilization conditions were achieved, otherwise the growth of the spores indicates that the sterilization process has not been met.
Recently, a DNA polymerase derived from these bacteria, Bst polymerase, has become important in molecular biology applications.
Bst polymerase has a helicase-like activity, making it able to unwind DNA strands. Its optimum functional temperature is between 60 and 65°C and it is denatured at temperatures above 70°C. These features make it useful in loop-mediated isothermal amplification (LAMP). LAMP is similar to the polymerase chain reaction (PCR) but does not require the high temperature (96°C) step required to denature DNA.
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