Cytokinesis

Cytokinesis

Cilliate undergoing cytokinesis, with the cleavage furrow being clearly visible.
Animal cell telophase and cytokinesis

Cytokinesis (from the polar bodies, which then die. Another form of mitosis without cytokinesis occurs in the liver, yielding multinucleate cells. In plant cells, a dividing structure known as the cell plate forms within the centre of the cytoplasm and a new cell wall forms between the two daughter cells.

Cytokinesis is distinguished from the prokaryotic process of binary fission.

Contents

  • Animal cell cytokinesis 1
    • Contractile ring positioning 1.1
    • Timing cytokinesis 1.2
  • Plant cell cytokinesis 2
  • Etymology and pronunciation 3
  • See also 4
  • References 5
  • Further reading 6

Animal cell cytokinesis

Contractile ring positioning

During different, proliferative divisions, animal cell cytokinesis begins shortly after the onset of sister interphase (see also cell cycle).

The position at which the contractile ring assembles is dictated by the mitotic spindle.[1] This seems to depend upon the GTPase RhoA, which influences several downstream effectors (such as the protein kinases ROCK and citron) to promote myosin activation (by influencing the phosphorylation of Myosin regulatory light chain (rMLC)) and actin filament assembly (by regulating formin protein) at a particular region of the cell cortex.[2]

Simultaneous with contractile ring assembly during prophase, a microtubule based structure termed the central spindle (or spindle midzone) forms when non-kinetochore microtubule fibres are bundled between the spindle poles. A number of different species including H. sapiens, D. melanogaster and C. elegans require the central spindle in order to efficiently undergo cytokinesis, although the specific phenotype described when it is absent varies from one species to the next (for example, certain Drosophila cell types are incapable of forming a cleavage furrow without the central spindle, whereas in both C. elegans embryos and human tissue culture cells a cleavage furrow is observed to form and ingress, but then regress before cytokinesis is complete). Seemingly vital for the formation of the central spindle (and therefore efficient cytokinesis) is a heterotetrameric protein complex called centralspindlin. Along with associated factors (such as SPD-1 in C. elegans), centralspindlin plays a role in bundling microtubules to form the spindle midzone during anaphase.

Timing cytokinesis

Cytokinesis must be temporally controlled to ensure that it occurs only after sister chromatids separate during the anaphase portion of normal proliferative cell divisions. To achieve this, many components of the cytokinesis machinery are highly regulated to ensure that they are able to perform a particular function at only a particular stage of the cell cycle.[3][4] Cytokinesis happens only after APC binds with CDC20. This allows for the separation of chromosomes and myosin to work simultaneously.

Plant cell cytokinesis

Due to the presence of a cell wall, cytokinesis in plant cells is significantly different from that in animal cells, Rather than forming a contractile ring, plant cells construct a cell plate in the middle of the cell. The stages of cell plate formation include (1) creation of the phragmoplast, an array of microtubules that guides and supports the formation of the cell plate; (2) trafficking of vesicles to the division plane and their fusion to generate a tubular-vesicular network; (3) continued fusion of membrane tubules and their transformation into membrane sheets upon the deposition of callose, followed by deposition of cellulose and other cell wall components; (4) recycling of excess membrane and other material from the cell plate; and (5) fusion with the parental cell wall [5][6]

The phragmoplast is assembled from the remnants of the mitotic spindle, and serves as a track for the trafficking of vesicles to the phragmoplast midzone. These vesicles contain lipids, proteins and carbohydrates needed for the formation of a new cell boundary. Electron tomographic studies have identified the Golgi apparatus as the source of these vesicles,[7][8] but other studies have suggested that they contain endocytosed material as well.[9][10]

These tubules then widen and fuse laterally with each other, eventually forming a planar, fenestrated sheet [8]. As the cell plate matures, large amounts of membrane material are removed via clathrin-mediated endocytosis [7] Eventually, the edges of the cell plate fuse with the parental plasma membrane, often in an asymmetrical fashion,[11] thus completing cytokinesis. The remaining fenestrae contain strands of endoplasmic reticulum passing through them, and are thought to be the precursors of plasmodesmata [8].

The construction of the new cell wall begins within the lumen of the narrow tubules of the young cell plate. The order in which different cell wall components are deposited has been determined largely by immuno-electron microscopy. The first components to arrive are pectins, hemicelluloses, and arabinogalactan proteins carried by the secretory vesicles that fuse to form the cell plate.[12] The next component to be added is callose, which is polymerized directly at the cell plate by callose synthases. As the cell plate continues to mature and fuses with the parental plasma membrane, the callose is slowly replaced with cellulose, the primary component of a mature cell wall [6]. The middle lamella (a glue-like layer containing pectin) develops from the cell plate, serving to bind the cell walls of adjoining cells together.[13][14]

Etymology and pronunciation

The word cytokinesis () uses combining forms of cyto- + kine- + -sis, New Latin from Classical Latin and Ancient Greek, reflecting "cell" and kinesis ("motion, movement").

See also

References

  1. ^ Rappoport R: "Cytokinesis in Animal Cells", Cambridge University Press (1996)
  2. ^ Glotzer, M: "Animal cell cytokinesis", Annual Review of Cell Biology 17, 351-86 (2001)
  3. ^ J. Mishima et al.: "Cell cycle regulation of central spindle correctly", Nature 430, 908-913 (2004)
  4. ^ Petronczki et al.: "Polo-like kinase 1 triggers the initiation of cytokinesis in human cells by promoting recruitment of the RhoGEF Ect2 to the central spindle", Developmental Cell 12, 713-725 (2007)
  5. ^ Otegui, M., and Staehelin, L.A. "Cytokinesis in flowering plants: more than one way to divide a cell." Curr. Opin. Plant Biol. 3, 493-502 (2000)
  6. ^ Samuels, A.L., Giddings,T.H.Jr., and Staehelin, L.A. "Cytokinesis in tobacco BY-2 and root tip cells: a new model of cell plate formation in higher plants." J. Cell Biol. 130, 1345-1357 (1995).
  7. ^ Otegui, M.S., Mastronarde, D.N., Kang, B.H., Bednarek, S.Y., and Staehelin, L.A. "Three-dimensional analysis of syncytial-type cell plates during endosperm cellularization visualized by high resolution electron tomography." Plant Cell 13, 2033-2051 (2001)
  8. ^ Segui-Simarro, J.M., Austin, J.R.,2nd, White, E.A., and Staehelin, L.A. "Electron tomographic analysis of somatic cell plate formation in meristematic cells of Arabidopsis preserved by high-pressure freezing." Plant Cell 16, 836-856 (2004)
  9. ^ Baluška, F., Liners, F., Hlavačka, A., Schlicht, M., Van Cutsem, P., McCurdy, D.W., and Menzel, D. "Cell wall pectins and xyloglucans are internalized into dividing root cells and accumulate within cell plates during cytokinesis." Protoplasma 225, 141-55 (2005)
  10. ^ Dhonukshe, P., Baluška, F., Schlicht, M., Hlavacka, A., Šamaj, J., Friml, J., and W., G.T.,Jr. "Endocytosis of cell surface material mediates cell plate formation during plant cytokinesis." Dev. Cell 10, 137-50 (2006)
  11. ^ Cutler, S.R., and Ehrhardt, D.W. "Polarized cytokinesis in vacuolate cells of Arabidopsis." Proc. Natl. Acad. Sci. USA 99, 2812-2817 (2002)
  12. ^ Staehelin, L.A., and Moore, I. "The Plant Golgi Apparatus: Structure, Functional Organization and Trafficking Mechanisms." Annu. Rev. Plant Physiol. Plant Mol. Biol. 46, 261-288 (1995)
  13. ^ Charles E. Allen (July 1901). "On the Origin and Nature of the Middle Lamella". Botanical Gazette 32 (1): 1–34.  
  14. ^ Eichorn, Susan, et al. Esau's Plant Anatomy: Meristems, Cells, and Tissues of the Plant Body: Their Structure, Function, and Development, 3rd Edition. 2006. ISBN 978-0-471-73843-5

Further reading

  • The Molecular Requirements for Cytokinesis by M. Glotzer (2005), Science 307, 1735
  • "Animal Cytokinesis: from parts list to mechanism" by Eggert, U.S., Mitchison, T.J., Field, C.M. (2006), Annual Review of Cell Biology 75, 543-66
  • Campbell Biology (2010), 580-582
  • More description and nice images of cell division in plants, with a focus on fluorescence microscopy
  • Nanninga, Nanne. Cytokinesis in Prokaryotes and Eukaryotes: Common Principles and Different Solutions